Review



silentfect lipid reagent  (Bio-Rad)


Bioz Verified Symbol Bio-Rad is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Bio-Rad silentfect lipid reagent
    Silentfect Lipid Reagent, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 420 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/silentfect+lipid+reagent/siLentFect+Lipid+Reagent+for+RNAi/pmc13083780-209-7-10
    Average 96 stars, based on 420 article reviews
    silentfect lipid reagent - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Transfection:

    Article Title: METTL1-WDR4 promotes the migration and proliferation of gastric cancer through N 7 -methylguanosine.
    Article Snippet: Negative control siRNA (si–NC), METTL1 (si–M1), WDR4 (si–W4), YY1, PIK3C2B, AKT siRNA, were purchased from Tsingke Biotech (Beijing, China). .. Cells were transfected with siRNA at 30–50% confluence using siLentFect Lipid Reagent (Bio-Rad, CA, USA). .. METTL1, WDR4, YY1, PIK3C2B, AKT were amplified from human cDNA template and cloned into the pcDNA3.1(+) vector (Invitrogen, USA).

    Article Title: Nuclear Lipin1 recruits HDAC2 to epigenetically repress SREBP1-dependent lipid synthesis and myelination in hypoxic preterm white matter injury.
    Article Snippet: .. Media were changed every 3 days, and cells were differentiated for 5–10 days. siRNA transfection OPCs were transfected at 60%–70% confluency using SilentFect Lipid Reagent (Bio-Rad, 1703361). siRNAs targeting Lipin1 or HDAC2 (sequences in Table S1) were diluted in serum-free DMEM/F-12 and incubated with lipid reagent for 20 min before addition to cells. ..

    Article Title: Transient receptor potential vanilloid four in macrophages mediates TGF-β activation to drive myofibroblast differentiation and pulmonary fibrosis
    Article Snippet: BMDM CM (from 500,000 BMDM/ml) was added directly to attached MLEC for 20 h, or, for coculture experiments, MLEC and BMDM were plated directly together for 20 h. MLECs were lysed, and luminescence was determined with a luciferase assay kit (Promega) and luminometer (SpectraMax iD3, Molecular Devices, Softmax Pro Software 7.0.3). .. All siRNAs were transfected into WT MLFs using siLentFect lipid reagent (Bio-Rad) according to the manufacturer’s instructions. .. SMAD2-or SMAD3-specific siRNA and control scrambled siRNA duplexes were purchased from Dharmacon and used at the indicated concentrations (24 h of transfection).

    Article Title: EZH2 Inhibition Induces an Integrated Stress Response Driving Glutamine-Dependent Vulnerability in TNBC
    Article Snippet: Horseradish peroxidase–conjugated (HRP) secondary anti-mouse and anti-rabbit IgG were used for 45 minutes to allow immunodetection using Clarity Western ECL substrate (BioRad) for enhanced chemiluminescence (PerkinElmer Life and Analytical Sciences). .. Cells were seeded and transfected 4 hours later with siRNA (20 nM) using the siLentFect lipid reagent (BioRad) according to the manufacture’s protocol for a total duration of 48-72 hours. ..

    Article Title: Transient Receptor Potential Vanilloid 4 in Macrophages Mediates TGF-β Activation to Drive Myofibroblast Differentiation and Pulmonary Fibrosis.
    Article Snippet: 420 MLECs were lysed, and luminescence was determined with a luciferase assay kit (Promega) and 421 luminometer (SpectraMax iD3, Molecular Devices, Softmax Pro Software 7.0.3). .. 422 423 siRNA mediated knockdown 424 All siRNAs were transfected into WT MLFs using siLentFect lipid reagent (Bio-Rad) according 425 to the manufacturer’s instructions. .. SMAD2- or SMAD3-specific siRNA and control scrambled 426 siRNA duplexes were purchased from Dharmacon and used at the indicated concentrations (24 427 hours of transfection).

    Article Title: Nuclear Lipin1 recruits HDAC2 to epigenetically repress SREBP1-dependent lipid synthesis and myelination in hypoxic preterm white matter injury
    Article Snippet: .. OPCs were transfected at 60%–70% confluency using SilentFect Lipid Reagent (Bio-Rad, 1703361). siRNAs targeting Lipin1 or HDAC2 (sequences in ) were diluted in serum-free DMEM/F-12 and incubated with lipid reagent for 20 min before addition to cells. ..

    Article Title: CircLPP Activates the Wnt/β-Catenin Signaling Pathway via the miR-665/Wnt3a Axis and Promotes Proliferation and Metastasis in Colorectal Cancer.
    Article Snippet: Circular RNAs (circRNAs) are covalently closed RNA molecules that play critical roles in tumorigenesis and cancer progression, including colorectal cancer (CRC).. However, the clinical significance, biological functions, and molecular mechanisms of many novel circRNAs in CRC remain poorly understood.. In this study, we identified a novel circRNA, hsa_circ_0003759 (designated circLPP), which was significantly upregulated in CRC tissues.

    Article Title: Antisense oligonucleotides targeting transcription termination windows disrupt mRNA 3’ end processing and decrease gene expression
    Article Snippet: .. Transfections were performed with siLentFect lipid reagent (BioRad) according to the manufacturer’s instructions, with siRNAs at a final concentration of 20 nM. siRNAs used were ON-TARGETplus SMARTpool (Horizon). .. RNA isolation was performed using RNeasy kit (Qiagen).

    Incubation:

    Article Title: Nuclear Lipin1 recruits HDAC2 to epigenetically repress SREBP1-dependent lipid synthesis and myelination in hypoxic preterm white matter injury.
    Article Snippet: .. Media were changed every 3 days, and cells were differentiated for 5–10 days. siRNA transfection OPCs were transfected at 60%–70% confluency using SilentFect Lipid Reagent (Bio-Rad, 1703361). siRNAs targeting Lipin1 or HDAC2 (sequences in Table S1) were diluted in serum-free DMEM/F-12 and incubated with lipid reagent for 20 min before addition to cells. ..

    Article Title: Nuclear Lipin1 recruits HDAC2 to epigenetically repress SREBP1-dependent lipid synthesis and myelination in hypoxic preterm white matter injury
    Article Snippet: .. OPCs were transfected at 60%–70% confluency using SilentFect Lipid Reagent (Bio-Rad, 1703361). siRNAs targeting Lipin1 or HDAC2 (sequences in ) were diluted in serum-free DMEM/F-12 and incubated with lipid reagent for 20 min before addition to cells. ..

    Knockdown:

    Article Title: Transient Receptor Potential Vanilloid 4 in Macrophages Mediates TGF-β Activation to Drive Myofibroblast Differentiation and Pulmonary Fibrosis.
    Article Snippet: 420 MLECs were lysed, and luminescence was determined with a luciferase assay kit (Promega) and 421 luminometer (SpectraMax iD3, Molecular Devices, Softmax Pro Software 7.0.3). .. 422 423 siRNA mediated knockdown 424 All siRNAs were transfected into WT MLFs using siLentFect lipid reagent (Bio-Rad) according 425 to the manufacturer’s instructions. .. SMAD2- or SMAD3-specific siRNA and control scrambled 426 siRNA duplexes were purchased from Dharmacon and used at the indicated concentrations (24 427 hours of transfection).

    Concentration Assay:

    Article Title: Antisense oligonucleotides targeting transcription termination windows disrupt mRNA 3’ end processing and decrease gene expression
    Article Snippet: .. Transfections were performed with siLentFect lipid reagent (BioRad) according to the manufacturer’s instructions, with siRNAs at a final concentration of 20 nM. siRNAs used were ON-TARGETplus SMARTpool (Horizon). .. RNA isolation was performed using RNeasy kit (Qiagen).



    Similar Products

    96
    Bio-Rad silentfect lipid reagent
    Silentfect Lipid Reagent, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/silentfect+lipid+reagent/siLentFect+Lipid+Reagent+for+RNAi/pmc13083780-209-7-10
    Average 96 stars, based on 1 article reviews
    silentfect lipid reagent - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Bio-Rad rnai bio rad
    Rnai Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/silentfect+lipid+reagent/siLentFect+Lipid+Reagent+for+RNAi/pm41932313-993-182-183
    Average 96 stars, based on 1 article reviews
    rnai bio rad - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Bio-Rad silentfect lipid
    Silentfect Lipid, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/silentfect+lipid+reagent/siLentFect+Lipid+Reagent+for+RNAi/pm41932313-993-178-183
    Average 96 stars, based on 1 article reviews
    silentfect lipid - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Bio-Rad silentfect
    Silentfect, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/silentfect+lipid+reagent/siLentFect+Lipid+Reagent+for+RNAi/pm41910365-310-39-43
    Average 96 stars, based on 1 article reviews
    silentfect - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Bio-Rad silenfect lipid reagent
    Silenfect Lipid Reagent, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/silentfect+lipid+reagent/siLentFect+Lipid+Reagent+for+RNAi/pmc12997302-361-36-39
    Average 96 stars, based on 1 article reviews
    silenfect lipid reagent - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Bio-Rad silentfecttm lipid reagent for rnai
    Silentfecttm Lipid Reagent For Rnai, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/silentfect+lipid+reagent/siLentFect+Lipid+Reagent+for+RNAi/pmc12997302-55-0-6
    Average 96 stars, based on 1 article reviews
    silentfecttm lipid reagent for rnai - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Bio-Rad silentfect reagent
    Silentfect Reagent, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/silentfect+lipid+reagent/siLentFect+Lipid+Reagent+for+RNAi/pmc12972116-280-29-32
    Average 96 stars, based on 1 article reviews
    silentfect reagent - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    Image Search Results